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In rodents xenobiotics causing peroxisome proliferation induce enzymes of the peroxisomal -oxidation and also the microsomal cytochrome P450 4A family or CYP 4A family (4A1, 4A2, 4A3) involved in the -oxidation of fatty acids (Bell et al, 1992; Muerhoff et al, 1992) Peroxisome proliferator response elements (PPREs) have been reported for rodent cytochrome P450 4A6 and P450 4A1 (Muerhoff et al, 1992; Aldridge et al, 1995; reviewed by Simpson, 1997) Intraperitoneal injection of peroxisome proliferators (the hypolipidemic drugs clo brate or cipro brate) into bluegill (Lepomis macrochirus) and cat sh (Ictalurus punctatus), causes induction of both CYP2M1 and CYP2K1 cytochrome P450 isozymes, known to be associated with lauric acid hydroxylase activity (Haasch, 1996; Haasch et al, 1998) As in mammals, induction was sex-speci c, the protein being more inducible in male bluegill liver and in male cat sh kidney and possibly liver 212.

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3 Peroxisome proliferation Peroxisomes are membrane-bound cytoplasmic organelles appearing in most eukaryotic cells (Hruban & Rechcigl, 1969) Although the enzyme composition of peroxisomes is variable depending on the species, organ or cell type studied, all peroxisomes contain a variety of H2O2 producing oxidases and catalase, which degrades H2O2 (de Duve, 1965; Hruban & Rechcigl, 1969; B ck et al, 1980; Fahimi & Cajaraville, 1995) Apart from their pivotal role in oxyradical metabolism, peroxisomes are involved in several aspects of lipid metabolism These include -oxidation of long chain and very long chain fatty acids, bile acid formation, biosynthesis of ether lipids, biosynthesis of cholesterol and dolichol, and catabolism of prostaglandins and leukotrienes (Reddy & Mannaerts, 1994; Singh, 1997) One of the unique features of peroxisomes is their ability to undergo a massive proliferation, a phenomenon termed peroxisome proliferation which is induced by a number of endogenous compounds and xenobiotics.

Peroxisome proliferation consists of an increase in peroxisome number and fractional volume This is usually accompanied by the induction of some peroxisomal enzyme activities, particularly those of the fatty acid -oxidation system (Reddy & Mannaerts, 1994) Acyl-CoA oxidase (AOX), the enzyme catalysing the rst reaction of the -oxidation pathway, the multifunctional enzyme (enoyl-CoA hydratase/3hydroxyacyl-CoA dehydrogenase/isomerase or PH) and thiolase (3-ketoacyl-CoA thiolase.

The calculation of the GDOP and various other DOP are then de ned in terms of this H t matrix as a function of time t: A t H t T H t 1 ; p GDOP t tr A t ; q PDOP t A t 1;1 A t 2;2 A t 3;3 ; q HDOP t A t 1;1 A t 2;2 ; q VDOP t A t 3;3 ; q TDOP t A t 4;4 : 7.8.5 Implementation Results

of others allows greater acceptance Partners need to gain knowledge of the systemic nature of gender socialization and appreciate the plight and pain of both genders The therapist must create an environment of safety in which clients can discard their defensiveness and entitlement and develop true empathy for their partner This safe environment necessitates that both partners must feel heard, understood, and valued It is best at first to avoid toxic and emotionally laden topics on which the couple disagree and that have caused interpersonal stress One helpful way to gain distance from current pain and problems is to focus on the past to discuss gender messages they received from parents, extended family, teachers, peers, media, and others from childhood, with the goal to connect these old messages and expectations to present behaviors and attitudes.

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